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Primary Antibodies
[KO Validated] Cyclin B1 Rabbit mAb
[KO Validated] Cyclin B1 Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M0562
Reactivity:Human,Mouse
Applications:WB,ELISA
Modifcation:Unmodified
Source:Rabbit
Storage and Stability:Store at -20℃/ 1 year. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20ul Size Available
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[KO Validated] Cyclin B1 Rabbit mAb

Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit mAb (A19037) at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 45s.

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Product Details
Product Name
[KO Validated] Cyclin B1 Rabbit mAb
Description
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
Catalog No
M0562
Reactivity
Human, Mouse
Applications
WB, ELISA
Application Dilutions
WB,1:2500 - 1:10000 | ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Modifcation
Unmodified
Source
Rabbit
Isotype
IgG
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 3-177 of human Cyclin B1 (P14635).
Calculated MW (kDa)
48kDa
Observed MW (kDa)Gene Name
55kDaCCNB1
Other Name
CCNB; B1
Gene ID
891
Human Swiss Prot
P14635
Formulation
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Storage and Stability
Store at -20℃/ 1 year. Avoid freeze / thaw cycles.
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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